203325

BMS

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.

• Sinónimos: BMS, bis(2-Mercaptoethylsulfone)
• Fórmula empírica (notación de Hill): C₄H₁₀O₂S₃
• Número de CAS: 145626-87-5
• Peso molecular: 186.32
• Código UNSPSC: 12352128

Propiedades

• Nivel de calidad: 100
• Análisis: ≥99% (titration)
• formulario: crystalline solid
• idoneidad de la reacción: reagent type: reductant
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• color: white
• pKa: (1) 7.9, (2) 9.0
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• InChI: 1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
• Clave InChI: YWXCBLUCVBSYNJ-UHFFFAOYSA-N

Descripción

Descripción general

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pK_a of 7.9 and 9.0.

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at with BMS than with DTT at pH 7.0.

Envase

Packaged under inert gas

Advertencia

Toxicity: Standard Handling (A)

Nota de preparación

May require warming (40-45°C) to achieve complete solubilization in H₂O.

Otras notas

Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable