1.24546

Fluorescein-5-isothiocyanate (FITC)

for biochemistry and for fluorescence microscopy

• Sinónimos: Fluorescein-5-isothiocyanate (FITC)
• Fórmula empírica (notación de Hill): C₂₁H₁₁NO₅S
• Número de CAS: 3326-32-7
• Peso molecular: 389.38
• Número MDL: MFCD00005063
• Código UNSPSC: 12171501
• Número índice de la CE: 222-042-0

Propiedades

• Nivel de calidad: 400
• formulario: solid
• composición: Dye, ≥95% spectrophotometric assay
• mp: >360 °C
• εmax: 2200-2380 at 488-494 nm in 0.1 M NaOH at 0.003 g/L
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clave InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Descripción

Descripción general

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Nota de análisis

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°C): Not applicable