SRP8023
PARG human
recombinant, expressed in Sf21 cells, His tagged, >95% (SDS-PAGE)
Sinónimos:
FNDC5 (cleaved), Poly(ADP-ribose) glycohydrolase
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About This Item
Productos recomendados
origen biológico
human
recombinante
expressed in Sf21 cells
etiqueta
His tagged
Ensayo
>95% (SDS-PAGE)
Formulario
liquid
mol peso
~110 kDa by SDS-PAGE
envase
pkg of 2 μg
concentración
≥0.2 mg/mL
técnicas
cell based assay: suitable
color
clear
solubilidad
water: soluble
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... PARG(8505)
Descripción general
Research area: Cell signaling
Aplicación
PARG human has been used in the digestion of sciatic nerve sections, in co-immunoprecipitation, in in vitro glycohydrolase activity assay
Acciones bioquímicas o fisiológicas
Poly(ADP-ribose) glycohydrolase(PARG) hydrolyzes poly(ADP-ribose) at glycosidic(1′′-2′′) linkage of ribose-ribose bond to produce free ADP-ribose. It is an endo- and exo-glycosidase. PARG plays arole in DNA repair and replication. Inhibited or depleted PARG in cells aremore prone to DNA-damaging agents. PARG also averts the accumulation of PAR inthe cytoplasm and parthanatos, a caspase-independent PAR-mediated type of celldeath.
Forma física
Solution in 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2% NP-40, 50 mM imidazole and 10% glycerol.
Otras notas
Human PARG is fused to a His-tag.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Repr. 1B
Código de clase de almacenamiento
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Daniel Harrision et al.
Frontiers in molecular biosciences, 7, 191-191 (2020-10-03)
Poly(ADP-ribose) polymerases (PARPs) are a family of enzymes that catalyze the addition of poly(ADP-ribose) (PAR) subunits onto themselves and other acceptor proteins. PARPs are known to function in a large range of cellular processes including DNA repair, DNA replication, transcription
Sara C Larsen et al.
Cell reports, 24(9), 2493-2505 (2018-08-30)
ADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirm that serine residues are
Sara C Buch-Larsen et al.
Cell reports, 32(12), 108176-108176 (2020-09-24)
ADP-ribosylation (ADPr) is a post-translational modification that plays pivotal roles in a wide range of cellular processes. Mass spectrometry (MS)-based analysis of ADPr under physiological conditions, without relying on genetic or chemical perturbation, has been hindered by technical limitations. Here
Xiao-Nan Zhang et al.
Nature communications, 10(1), 4196-4196 (2019-09-15)
Nicotinamide adenine dinucleotide (NAD+)-dependent ADP-ribosylation plays important roles in physiology and pathophysiology. It has been challenging to study this key type of enzymatic post-translational modification in particular for protein poly-ADP-ribosylation (PARylation). Here we explore chemical and chemoenzymatic synthesis of NAD+
Jeannette Abplanalp et al.
Nature communications, 8(1), 2055-2055 (2017-12-14)
ADP-ribosylation is a posttranslational modification that exists in monomeric and polymeric forms. Whereas the writers (e.g. ARTD1/PARP1) and erasers (e.g. PARG, ARH3) of poly-ADP-ribosylation (PARylation) are relatively well described, the enzymes involved in mono-ADP-ribosylation (MARylation) have been less well investigated.
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