This kit was validated for use with serum, plasma and whole blood. Cell lysates have not been analyzed with this kit and no specific guidance is provided for this type of sample preparation.
MAK316
Heme Assay Kit
sufficient for 250 colorimetric tests
Sinónimos:
Heme Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
detection method
colorimetric
relevant disease(s)
hematological disorder
storage temp.
2-8°C
General description
Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.[1][2]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.
Principle
Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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How does this kit work with cell culture sample? How do you homogenize the cells prior of measurement?
1 answer-
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Could I use distilled water instead of ultrapure? Can I use this kit to measure how much plasma free hemoglobin I have in a plasma sample?
1 answer-
Only ultra pure water should be used because it is filtered through a 0.22 μm filter which removes any bacteria that could interfere with the chemical reaction.
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Does MAK316 work for mouse sera?
1 answer-
MAK316 is expected to work for all species and should be compatible with mouse sera samples, as well as with other sample types such as blood, serum, plasma, and urine.
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how is this kit used
1 answer-
The kit is used according to the product protocol. Here is a link to that document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/386/102/mak316bul.pdf
Helpful?
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お世話になります。臨床研究でヒトの血栓を回収してホルマリン固定しております。遊離ヘムを染めたいと考えておりますが 可能でしょうか？また、検体を送れば染めていただけるのでしょうか？
1 answer-
This product is specifically tested for the quantitative determination of heme in various biological samples. Solid tissues are homogenized and lysed by the heme reagent which contains NaOH and a detergent. This would also apply to the clot sample resulting in the lysis of any intact RBCs. Given the principle of the kit, measuring only the free heme would not be possible. Currently, there is no product available to meet your requirements. Unfortunately, lab services are not offered for this particular application.
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