The solubility of this product has been tested in water at a concentration of 20 mg/mL, resulting in dark brown-red solutions. Unfortunately, we have not tested this product in colorimetric assays and can provide no recommendations on this.
H2500
Hemoglobin from bovine blood
lyophilized powder
Sinónimos:
Bovine hemoglobin, Hb, Methemoglobin
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Form:
lyophilized powder
Biological source:
bovine blood
Mol wt:
Mr ~64500
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Servicio técnico
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Permítanos ayudarlebiological source
bovine blood
Quality Segment
form
lyophilized powder
mol wt
Mr ~64500
technique(s)
MALDI-TOF: suitable
storage temp.
2-8°C
Gene Information
General description
Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with each 141 and 146 amino acids, respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.
Application
The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.
Hemoglobin from bovine blood has been used in:
- standard curve generation for the quantification intraparenchymal hemorrhage and parenchymal hemorrhage in spinal cord homogenate using Drabkin′s assay, Quadrupole-Ion Mobility-Time-of-Flight mass spectrometery
- the generation of molecularly imprinted polymers (MIPs) to mimic high molecular-weight polyethylene glycol (PEG) in crystallization studies
Biochem/physiol Actions
Oxygen transporter, NO scavenger
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.
Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide. Bovine hemoglobin is used in the generation of hemoglobin-vesicles (HbV) for oxygen transport. It is thermally stable and displays high affinity to oxygen when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine haemoglobin is recommended for treating autoimmune hemolytic anemia.
Disclaimer
Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.
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Este artículo | |||
|---|---|---|---|
| technique(s) MALDI-TOF: suitable | technique(s) activity assay: suitable | technique(s) - | technique(s) - |
| biological source bovine blood | biological source bovine blood | biological source bovine blood | biological source - |
| form lyophilized powder | form substrate powder | form dried erythrocytes | form lyophilized |
| mol wt Mr ~64500 | mol wt Mr ~64500 | mol wt Mr ~64500 | mol wt - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I want to create hemoglobin standards to use in a colorimetric assay. Can I create hemoglobin concentrations by weighing specific amounts of lyophilized hemoglobin powder and reconstituting with TS saline? Then process in assay after adding reagent?
1 answer-
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Does the website specify if H2500 Hemoglobin from bovine blood is ferrohemoglobin or oxyhemoglobin?
1 answer-
The website indicates that the product H2500 is mainly methemoglobin. A search of the website did not uncover any products marketed as either ferrohemoglobin or oxyhemoglobin.
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What is the difference between H2500 and H2625 when making Chocolate agar with bovine hemoglobin? Although they share the same CAS and Specification Sheet, there is a significant difference in price between the two.
1 answer-
Products H2500 and H2625 are not suitability tested for use in producing Chocolate Agar. The key differences between H2500 and H2625 lie in their purity and intended uses. H2500 is a purer product, suitable for MALDI-TOF, and associated with a companion product for HPLC analysis of hemoglobin variants. It undergoes two steps of ammonium sulfate precipitation, filtration, dialysis, and lyophilization. On the other hand, H2625 is not suitability tested for use as a protease substrate and lacks the ammonium sulfate precipitation step. These distinctions likely contribute to the significant price difference between the two products.
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