This product can be dissolved in PBS and used at up to 3 ug/ml for mitogenic effects on human peripheral blood mononuclear cells. Any concentration higher than 3 ug/ml could be toxic to these cells.
L8777
Lectin from Phytolacca americana (pokeweed)
lyophilized powder, BioReagent, suitable for cell culture
Sinónimos:
PWM, Pokeweed mitogen
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About This Item
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esterilidad
aseptically filled
Nivel de calidad
Línea del producto
BioReagent
Formulario
lyophilized powder
potencia
≤0.3 μg/mL mitogenic activity
técnicas
cell culture | mammalian: suitable
solubilidad
PBS: 1 mg/mL
temp. de almacenamiento
2-8°C
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Descripción general
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.[1]
Aplicación
Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).[2]
Acciones bioquímicas o fisiológicas
Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.[3]
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Calidad
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Nota de análisis
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
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Artículos
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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how to prepare Pokeweed to be used as mitogen for B-ALL cytogenetics blood culture
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