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D8537

Sigma-Aldrich

Disolución salina tamponada con fosfatos de Dulbecco

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

product name

Disolución salina tamponada con fosfatos de Dulbecco, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

La disolución salina tamponada con fosfato de Dulbecco (DPBS) es una disolución salina equilibrada (BSS) utilizada para la manipulación y el cultivo de células de mamífero. El tampón DPBS se utiliza para irrigar, lavar y diluir las células de mamífero mientras se mantiene el equilibrio osmótico. Proporciona agua e iones inorgánicos esenciales para el metabolismo celular normal. El DPBS con calcio y magnesio facilita la unión y el agrupamiento celulares. El PBS de Dulbecco sin estos iones puede utilizarse para lavar y enjuagar las células suspendidas. El tampón de fosfato mantiene el pH en el intervalo fisiológico.

Application

La disolución salina tamponada con fosfato de Dulbecco se ha utilizado:

  • para la dilución de glóbulos rojos recién extraídos para estudiar la captura del agregado de eritrocitos.
  • para lavar los pocillos de una placa de microvaloración.
  • para lavar las células antes de resuspenderlas en tampón de lisis para el aislamiento del ADN
  • como componente del medio de conservación de embriones para la vitrificación de embriones.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

also commonly purchased with this product

Referencia del producto
Descripción
Precios

D1145

Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

D5796

Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

R8758

Medio RPMI-1640, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

F1051

Suero fetal bovino, Canada origin, sterile-filtered, suitable for cell culture

CLS430641

Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
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Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
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Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

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