The properties and content of this product have not been determined. The percentage of protein is lot specific. The Certificate of Analysis (CoA) provides information on the quality control release tests.
B4882
Bromelain from pineapple stem
≥3 units/mg protein
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
Specific activity:
≥3 units/mg protein
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Servicio técnico
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lyophilized powder
Quality Segment
specific activity
≥3 units/mg protein
composition
Protein, ≥30% biuret
storage temp.
−20°C
Application
Bromelain from pineapple stem has been used:
- to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
- as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
- to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Preparation Note
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Other Notes
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
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Este artículo | |||
|---|---|---|---|
| specific activity ≥3 units/mg protein | specific activity (chromatography purified), ≥4 units/mg protein | specific activity ≥125 units/mg protein | specific activity ≥100 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥30% biuret | composition Protein, 30-50% biuret | composition Protein, 40-70% | composition Protein, ≥20% Lowry |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I WILL USE BROMELAIN (B4882-10G ).IN A RESEARCH PROJECT. BROMELAIN IS COMPOSED OF MANY ENZYMES. THEY WERE ASKED WHAT the PROPERTIES AND CONTENT OF THE BROMELAIN YOU USE. I WOULD BE HAPPY IF YOU HELPED ME WITH
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