After the discontinous density gradient is prepared using Histopaque 10771 and Histopaque 1119, the tubes are centrifuged for 30 minutes at 700G. The Product Information Sheet is available from the website and includes the complete recommended procedure.
11191
Histopaque®-1119
sterile-filtered, density: 1.119
Histopaque®-1119
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NACRES:
NA.75
UNSPSC Code:
12352207
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Servicio técnico
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Permítanos ayudarlesterility
sterile-filtered
Quality Segment
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.119
application(s)
hematology
histology
storage temp.
2-8°C
General description
Histopaque®-1119 es un medio estéril, comprobado para endotoxinas y listo para usar. Esta disolución consiste en polisacarosa y diatrizoato de sodio, ajustados a una densidad relativa de 1,119 g/ml y a un pH de 8,8 a 9,0.
Application
Histopaque®-1119 es adecuado para utilizarlo junto con Histopaque-1077, ya que facilita la rápida recuperación de células mononucleares viables y de granulocitos de pequeños volúmenes de sangre completa o de médula ósea.
Biochem/physiol Actions
El proceso implica la formación de un gradiente doble al colocar una capa de igual volumen de Histopaque-1077 sobre el Histopaque-1119. La sangre completa anticoagulada se coloca con cuidado en capas sobre el medio Histopaque-1077 superior. Tras la centrifugación, la polisacarosa agrega los eritrocitos que, por tanto, sedimentan rápidamente. Los granulocitos se encuentran en la interfaz Histopaque-1077/1119 inferior, mientras que los linfocitos y otras células mononucleares se encuentran en la interfaz plasma/Histopaque-1077 superior. La mayoría de las plaquetas extrañas que se encuentran en la interfaz superior se quitan mediante centrifugación a baja velocidad durante los pasos de lavado. La contaminación con eritrocitos es insignificante.
Features and Benefits
Histopaque®-1119 ofrece numerosas ventajas, como:
- Recuperación óptima de células viables
- Permite la separación selectiva de los tipos de células sanguíneas
- Evita la distorsión celular
- Preserva la viabilidad celular
- Mínima interferencia de células extrañas
- Rendimiento reproducible de lote a lote
- Esterilización mediante filtración y probado para endotoxinas
- Estable a 2-8°C durante al menos 2 años cuando está protegido de la luz
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility aseptically filled |
| form liquid | form liquid | form liquid | form solution |
| density 1.119 | density 1.077 | density 1.083 | density 1.077 g/mL |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Clase de almacenamiento
10 - Combustible liquids
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 251429-50G | 04061838138613 |
| 251429-10G | 04061838138606 |
| 11191-6X100ML | 04061838260659 |
| 11191-100ML | 04061838699305 |
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Bei wieviel g muss das geschichtete (1,1077/1,119/Blut) und wie lange zentrufugiert werden um eine Trennung zu erreichen?
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 answer-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 answer-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
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Is there a product similar to Mono-Poly resolving media?
1 answer-
The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
1 answer-
Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
1 answer-
This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Helpful?
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 answer-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
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No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
1 answer-
Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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