Percoll is produced by Cytiva, formerly known as GE Life Sciences. Details regarding the proprietary manufacturing process are not publicly available. However, it can be mentioned that the Percoll particles range in size from 15 to 30 nanometers. While the density of Percoll solution is measured, the specific information regarding the amount of coating on the silica particles is not provided.
P1644
Percoll®
pH 8.5-9.5 (20 °C)
Sinónimos:
Medios de gradiente de densidad Percoll
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Servicio técnico
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Permítanos ayudarlesterility
aseptically filled
Quality Segment
form
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
technique(s)
cell culture | mammalian: suitable
color
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
application(s)
hematology
histology
storage temp.
2-8°C
General description
Percoll® es un medio clásico para centrifugación en gradiente de densidad de células, virus y partículas celulares. Percoll consiste en partículas de sílice coloidal de 15– 30 nm de diámetro (23% p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP). La PVP hace que el producto sea completamente atóxico e ideal para utilizar con materiales biológicos.
Application
Se ha utilizado Percoll® para la preparación del esperma en estudios de los requisitos de energía de los embriones bovinos.
El Percoll consiste en partículas de sílice coloidales de 15 a 30 nm de diámetro 23 % p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP. Se utiliza en la separación celular y en el aislamiento de orgánulos.
El Percoll consiste en partículas de sílice coloidales de 15 a 30 nm de diámetro 23 % p/p en agua) que han sido recubiertas con polivinilpirrolidona (PVP. Se utiliza para establecer gradientes de densidad no tóxicos con baja viscosidad y poca osmolaridad que pueden utilizarse para aislar células, orgánulos y virus. Se ha utilizado para separar vesículas de la membrana interna y externa de E. coli. El Percoll es isosmótico en todo el gradiente, no tóxico y fácil de eliminar de los materiales purificados.
Legal Information
Percoll is a registered trademark of Cytiva
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Este artículo | |||
|---|---|---|---|
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | plant: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| pH 8.5-9.5 (20 °C) | pH 8.5-9.5 (25 °C) | pH 8.9 (20 °C) | pH - |
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Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the details about the manufacturing process of Percoll, specifically regarding the amount of PVP used during bead soaking or the approximate PVP concentration within Percoll?
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What is procedure for sperm preparation for ivf with percoll gradient
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Please note that this product is intended for research use only. This material is not tested for the isolation of sperm samples. The following reference may be helpful:
Chen. Y. et al., Int. J. Fertil., 37, 315-319 (1992).Please see the link below to review the product datasheet for additional information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdfHelpful?
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CAN I FREEZE PERCOLL (P1644)? I SEE IN OTHER REF THAT I CAN, BUT NOT IN THIS REF
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This material should not be stored frozen for long periods of time. As per the product information sheet, if stored at –20 °C, gradients form upon thawing, necessitating mixing the contents of the bottle before use. Please see the link below to review the product datasheet for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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Percoll (P1644) 을 gradient 만드는데 사용했는데요, P4937도 같은 용도로 사용 가능한가요?
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Yes, product P4937 can be used for the same use as product P1644.
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I often find crystals. Can I filter the solution before use? What filter size could I use without affecting the functionality of the product?
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Particles may be removed by filtration or low speed centrifugation. More information about the particles filtration can be found in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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When using Product P1644, Percoll®, what density should I use for my particular system?
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The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between Percoll® Products P4937 and P1644?
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Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Can you help me calculate gradients for Percoll®, Product P1644?
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The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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Is there a "best" salt solution to use with Product P1644, Percoll®?
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Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested. Sucrose is included to prevent aggregation. You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.
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