10065
αα-Amilasa from Aspergillus oryzae
powder, ~30 U/mg
Sinónimos:
1,4-α-D-glucano glucanohidrolasa
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About This Item
Número CE:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Productos recomendados
Formulario
powder
actividad específica
~30 U/mg
mol peso
Mr ~51000
temp. de almacenamiento
2-8°C
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Descripción general
Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).
Aplicación
α-Amylase has been used:
- as a control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity
- for the hydrolysis of starch to explore the the role of wheat starch in frozen dough
- to inhibit Staphylococcus aureus biofilms
Acciones bioquímicas o fisiológicas
Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.
Definición de unidad
1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).
Otras notas
Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer
For the determination of fats in foodcitation; Application in (selective) hydrolysis/condensation of glycosidic bondscitation.
For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Han Tao et al.
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