Unfortunately, there is no specific protocol for use with plant extracts. Please see the link below to review the protocol:
https://www.emdmillipore.com/US/en/product/Protease-Assay-Kit,EMD_BIO-539125#anchor_USP
539125
Protease Assay Kit
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About This Item
Código UNSPSC:
41116133
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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uso
sufficient for 200 tests
Nivel de calidad
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
protect from light
assay range
sensitivity: 10 ng/mL
entrada
sample type biological sample(s)
método de detección
colorimetric
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
A kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
This Protease Assay Kit is suitable for the quantitative determination of protease activity in biological samples. This kit has been tested for its suitability in detecting a wide variety of proteases, including serine proteases (trypsin, chymotrypsin, thrombin, plasmin, elastase, subtilisin), cysteine proteases (papain, cathepsin B) and acid proteases (thermolysin, pepsin). Each kit provides sufficient reagents for 200 assays. When used as recommended, this kit is ~100-fold more sensitive than either the casein-gel assay or casein A280 assay. The Protease Assay Kit can detect ~10 ng/ml of protease when a 24 h incubation is used. The sensitivity can be enhanced by up to 3-fold by using a fluorometer (excitation 490 nm and emission 525 nm) rather than a spectrophotometer to detect the proteolytic end products.
Componentes
A Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
Advertencia
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Especificaciones
Assay Time: 3 h
Almacenamiento y estabilidad
• FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
• Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
• Incubation buffer and assay buffer may be stored at 4°C or -20°C.
• Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
• Incubation buffer and assay buffer may be stored at 4°C or -20°C.
Otras notas
Twining, S.S. 1984. Anal. Biochem.143, 30.
Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Wiesner, R., et al. 1982. Anal. Biochem.121, 290.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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