PF0100

Protease Fluorescent Detection Kit

high sensitivity assay

• Sinónimos: Protease Assay Kit
• NACRES: NA.26
• UNSPSC Code: 12352202

Propiedades

• Quality Segment: 200
• shipped in: dry ice
• storage temp.: −20°C

Descripción

Application

Protease Fluorescent Detection Kit has been used:

• in the detection of proteolytic activity using casein based protease assay
• for testing the extracellular proteolytic activity of fungal strains
• for quantifying mitochondrial protease activity
• for measuring the protease production from soil solution

Fluorescent Protease Detection Kit Video Protocol

Detection limit: approx. 50 ng trypsin

Preparation Note

Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Solo componentes del kit

• Incubation buffer 25 mL
• FITC-casein solution 5 mL
• Assay buffer 200 mL
• Protease standard (trypsin) 20 μg
• FITC control 50 mg
• Trichloroacetic acid (TCA) solution 30 mL

Información de seguridad

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H314,H317,H334,H335,H410
• pcodes: P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• target_organs: Respiratory system
• Clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3