T1775

L-Thyroxine

powder, BioReagent, suitable for cell culture

• Synonyme(s) : 3,3′,5,5″-Tetraiodo-L-thyronine, 3-[4-(4-Hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-L-alanine, T₄
• Formule linéaire : HOC₆H₂(I)₂OC₆H₂(I)₂CH₂CH(NH₂)CO₂H
• Numéro CAS: 51-48-9
• Poids moléculaire : 776.87
• Numéro Beilstein : 2228515
• Numéro CE : 200-101-1
• Numéro MDL: MFCD00002595
• Code UNSPSC : 12352209
• eCl@ss : 32190102
• ID de substance PubChem : 24899990
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: synthetic
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Pureté: ≥98% (HPLC)
• Forme: powder
• Puissance: 5-50 ng/mL
• Technique(s): cell culture | mammalian: suitable
• Pf: 223 °C (dec.) (lit.)
• Solubilité: 4 M NH₄OH in methanol: 50 mg/mL, clear to hazy, yellow
• Conditions d'expédition: ambient
• Température de stockage: room temp
• Chaîne SMILES: N[C@@H](Cc1cc(I)c(Oc2cc(I)c(O)c(I)c2)c(I)c1)C(O)=O
• InChI: 1S/C15H11I4NO4/c16-8-4-7(5-9(17)13(8)21)24-14-10(18)1-6(2-11(14)19)3-12(20)15(22)23/h1-2,4-5,12,21H,3,20H2,(H,22,23)/t12-/m0/s1
• Clé InChI: XUIIKFGFIJCVMT-LBPRGKRZSA-N
• Informations sur le gène: human ... THRA(7067) , THRB(7068) , TTR(7276) ; mouse ... Cnr2(12802) ; rat ... Cnr1(25248)

Description

Description générale

Thyroxine (T₄) is a tetraiodo derivative of the p-hydroxyphenyl ether of tyrosine, which functions as growth factors or cell growth inhibitors. T₄ is used to treat hypothyroidism and thyroid carcinoma. It regulates energy metabolism.

Application

L-Thyroxine has been used to investigate its role in colorectal cancer cell lines HCT 116 (APC wild type) and HT-29 (APC mutant). It has also been used to evaluate its effects on pro-inflammatory tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), angiogenic vascular endothelial growth factor (VEGF), leukocyte adhesion, intercellular adhesion molecule 1 (ICAM-1) and E-selectin in human umbilical vein endothelial cells (HUVECs).

Actions biochimiques/physiologiques

L-Thyroxine (T₄) and triiodo-L-thyronine (T₃) are iodine-containing hormones produced from thyroglobulin in the thyroid follicular cells. The stimulation of metabolic rate and regulation of growth and development by these hormones appear to be due to their effects on DNA transcription and, thus, protein synthesis.

Notes préparatoires

For long term storage, keep stock solutions at -20° in working aliquots and avoid repeated freeze/thaw cycles. Solutions stored at 2-8 °C are stable for up to 30 days.

Reconstitution

To prepare 20 μg/ml stock solution, dissolve in 50 ml sterile culture medium per mg thyroxine.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H372
• Conseils de prudence: P260 - P264 - P270 - P314 - P501
• Classification des risques: STOT RE 1
• Organes cibles: Thyroid,Cardio-vascular system,Kidney
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)