Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

SRP0255

Sigma-Aldrich

eIF4E Active human

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonyme(s) :

CBP, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥70% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

51 kDa

Conditionnement

pkg of 100 μg

Concentration

>0.02 mg/mL

Technique(s)

cell based assay: suitable

Adéquation

suitable for molecular biology

Numéro d'accès NCBI

NM_001968

Numéro d'accès UniProt

P06730

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... EIF4E(1977)

Description générale

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.

Application

Suitable substrate for MNK1 kinase.

Actions biochimiques/physiologiques

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.

Forme physique

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.
Pyronnet S, et al.
The Embo Journal, 18(1), 270-279 (1999)
Eukaryotic translation initiation factor 4E (eIF4E) binding site and the middle one-third of eIF4GI constitute the core domain for cap-dependent translation, and the C-terminal one-third functions as a modulatory region.
Morino S, et al.
Molecular and Cellular Biology, 20(2), 468-477 (2000)
Eukaryotic Translation Initiation Factor 4E Is a Feed-Forward Translational Coactivator of Transforming Growth Factor ? Early Protransforming Events in Breast Epithelial Cells.
Decarlo L, et al.
Molecular and Cellular Biology, 35(15), 2597-2609 (2015)
Yao Zhan et al.
The Journal of investigative dermatology, 135(5), 1368-1376 (2015-01-24)
In eukaryotic cells, the rate-limiting component for cap-dependent mRNA translation is the translation initiation factor eIF4E. eIF4E is overexpressed in a variety of human malignancies, but whether it has a role in melanoma remains obscure. We hypothesized that eIF4E promotes

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique