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PKH67GL

Sigma-Aldrich

Kit de lieur cellulaire à fluorescence verte au PKH67 pour le marquage des membranes cellulaires à usage général

Distributed for Phanos Technologies

Synonyme(s) :

Green PKH membrane labeling kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Conditions de stockage

protect from light

Fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

Méthode de détection

fluorometric

Conditions d'expédition

ambient

Température de stockage

room temp

Application

Ce kit est destiné au marquage des membranes cellulaires à usage général. Le PKH67 possède une queue de carbone aliphatique plus longue que le PKH1 et le PKH2, deux autres colorants verts précédemment décrits pour le suivi cellulaire in vitro et in vivo. Sur la base de la plus grande longueur de la queue, des études internes ont constamment montré une réduction du transfert cellule-cellule pour le PKH67 par rapport au PKH2.
Une perte lente de fluorescence a été observée dans des études in vivo utilisant le PKH1 et le PKH2. Le PKH67 peut présenter des propriétés similaires puisque ce comportement semble être caractéristique des colorants de liaison des cellules vertes, mais pas des colorants de liaison des cellules rouges. La corrélation entre la rétention in vitro de la membrane cellulaire et la demi-vie de fluorescence in vivo dans des cellules non en division permet de prévoir une demi-vie de fluorescence in vivo de 10-12 jours pour la PKH67. D'autres colorants verts de liaison cellulaire ayant une demi-vie similaire ont été utilisés pour suivre le trafic des lymphocytes et des macrophages in vivo sur des périodes de 1 à 2 mois, ce qui suggère que le PKH67 sera également utile pour des études de suivi in vivo de durée moyenne.
Le kit de marquage cellulaire en fluorescence verte PKH67 pour le marquage universel des membranes cellulaires a été utilisé dans les applications suivantes :
  • marquage et analyse des exosomes expulsés par les cellules dans le cancer du sein triple négatif[1]
  • marquage des cellules apoptotiques[2] afin d'étudier le rôle joué par le récepteur de l'ανβ5 dans la liaison et l'internalisation des cellules apoptotiques[3]
  • imagerie en fluorescence[4]

Liaison

Pour obtenir des détails techniques supplémentaires sur les colorants fluorescents PKH et CellVue® destinés au marquage cellulaire, notamment une bibliographie complète, rendez-vous ici.

Informations légales

CellVue is a registered trademark of Phanos Technologies

Composants de kit seuls

Réf. du produit
Description
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Yu Jun T, et al.
Scientific Reports, 6, 39140-39140 (2016)
Dandan Ma et al.
Cells, 9(4) (2020-04-01)
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their
alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Afficher tous les articles scientifiques apparentés

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

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Questions

1–10 of 12 Questions  

  1. I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?

    1 answer

    1. Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/

      Helpful?

  2. What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?

    1 answer

    1. Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdf

      Alternate methods for excess dye removal have not been investigated.

      Helpful?

  3. Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.

    1 answer

    1. The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.

      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/exosome-labeling-pkh

      Helpful?

  4. Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH76 dye can label the cell.

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  5. What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?

    1 answer

    1. PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-celldye transfer for PKH67 as compared with PKH2.

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  6. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.

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  9. How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).

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  10. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

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1–10 of 12 Questions  

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