MIDI67

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

• Synonyme(s) : Green PKH membrane midi labeling kit
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.32

Propriétés

• Conditionnement: pkg of 1 kit
• Fabricant/nom de marque: Distributed for Phanos Technologies
• Conditions de stockage: protect from light
• Technique(s): flow cytometry: suitable
• Fluorescence: λ_ex 490 nm; λ_em 502 nm (PKH67 dye)
• Application(s): cell analysis; detection
• Méthode de détection: fluorometric
• Conditions d'expédition: ambient
• Température de stockage: room temp

Description

Description générale

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Application

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:

• labeling exosomes  
• extracellular vesicles (EVs)l
• normal and sickle red blood cells (RBCs)
• Raji cells

Liaison

For additional technical details on PKH and CellVue^® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informations légales

CellVue is a registered trademark of Phanos Technologies

Composants de kit seuls

• PKH67 Dye 2 x 0.1
• Diluent C 6 x 10

Informations sur la sécurité

• Pictogrammes: GHS02,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225,H319
• Conseils de prudence: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 57.2 °F - closed cup
• Point d'éclair (°C): 14 °C - closed cup