K3627
Kallikrein from porcine pancreas
≥40 units/mg protein
Synonyme(s) :
Kininogenase, Kininogenin
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About This Item
Produits recommandés
Description générale
Kallikrein exists as an inactive prokallikrein in the porcine pancreas. The porcine kallikrein gene region is localized on chromosome 6q12-q21.
Application
Kallikrein from porcine pancreas has been used:
- as a matrix metalloproteinase-9 (MMP-9) zymogen activator
- as a component of cell culture to test its effect on rat subventricular zone (SVZ) cells and oligodendrocyte progenitor cells (OPC) proliferation and survival
- as a model enzyme to track kinetic data and visual detection limits of hydrolysis by hydrolytic enzymes in the two-phases array
Actions biochimiques/physiologiques
Kallikrein active forms are generated by the enzymatic action of trypsin. It is a serine protease that mediates the activation of growth factors and substrates.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of Nα-benzoyl-L-arginine ethyl ester (BAEE) to Nα-benzoyl-L-arginine and ethanol per min at pH 8.7 at 25°C.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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S C Fernando et al.
Genomics, 89(3), 429-438 (2007-01-11)
Kallikreins belong to a family of serine proteases that are widespread throughout living organisms, expressed in diverse tissue-specific patterns, and known to have highly diverse physiological functions. The 15 human and 24 mouse kallikreins have been implicated in pathophysiology of
Generation of alpha- and beta-kallikreins from porcine pancreatic prokallikrein by the action of trypsin.
M Kamada et al.
Chemical & pharmaceutical bulletin, 36(12), 4891-4899 (1988-12-01)
M G Cotenescu et al.
Journal of biotechnology, 76(1), 33-41 (2000-04-28)
A new assay is described that monitors hydrolysis with the concurrent transfer of a solvatochromic dye across an oil-water barrier. Through the appropriate design, this transfer is accompanied by a 10(6) gain in fluorescence. This response can be used to
Gabriel Rosenblum et al.
Journal of the American Chemical Society, 129(44), 13566-13574 (2007-10-13)
Activation of matrix metalloproteinase zymogen (pro-MMP) is a vital homeostatic process, yet its molecular basis remains unresolved. Using stopped-flow X-ray spectroscopy of the active site zinc ion, we determined the temporal sequence of pro-MMP-9 activation catalyzed by tissue kallikrein protease
Duncan J Campbell et al.
Clinical and experimental pharmacology & physiology, 38(9), 623-631 (2011-07-09)
1. Aliskiren is a renin inhibitor with an IC(50) of 0.6 nmol/L for human renin, 4.5 nmol/L for mouse renin and 80 nmol/L for rat renin. 2. In the present study, we compared the effects of aliskiren (10 mg/kg per day)
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