GE28-9321-71
HIS Spintrap™ Kit
Cytiva 28-9321-71, pack of 1 kit
About This Item
Produits recommandés
Conditionnement
pack of 1 kit
Fabricant/nom de marque
Cytiva 28-9321-71
Conditions de stockage
(0.15% Kathon CG)
(20% Ethanol)
Matrice
highly cross-linked 6% agarose
Taille moy. des particules
34 μm
cleaning
2-14(Ni2+-stripped medium.)
Plage de travail
3-12(Ni2+-stripped medium.)
Capacité
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Température de stockage
2-8°C
Catégories apparentées
Description générale
Caractéristiques et avantages
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Composants
1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Composants de kit également disponibles séparément
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00FDS
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53FDS
Mention d'avertissement
Danger
Mentions de danger
Code de la classe de stockage
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
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Articles
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocoles
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
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