GE17-5268-02
Ni Sepharose™ High Performance
Cytiva 17-5268-02, pack of 100 mL
Synonyme(s) :
High Performance Ni Resin, Ni Sepharose
About This Item
Produits recommandés
Conditionnement
pack of 100 mL
Fabricant/nom de marque
Cytiva 17-5268-02
Matrice
highly cross-linked 6% agarose
Taille moy. des particules
34 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni²⁺-stripped medium.)
Capacité
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Catégories apparentées
Description générale
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Caractéristiques et avantages
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Stockage et stabilité
Remarque sur l'analyse
Informations légales
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Protocoles
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