VA-ES-BJ

94092303, human skin (bone marrow metastasis), Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: VA-ES-BJ, 94092303
• Source biologique: human skin (bone marrow metastasis)
• Mode de croissance: Adherent
• Caryotype: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• Morphologie: Epithelial
• Produits: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• Récepteurs: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human skin epithelioid sarcoma

Description de la lignée cellulaire

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Application

Study of tumours. Tumourigenic in nude mice.

Milieu de culture

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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