MEWO

93082609, human skin, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: MEWO, 93082609
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Melanin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human malignant melanoma

Description de la lignée cellulaire

Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.

Application

Virus studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO₂; 37°C.

Autres remarques

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