C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Synonyme(s) :
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
Electrophorus electricus
Niveau de qualité
Type
Type V-S
Forme
lyophilized powder
Activité spécifique
≥1,000 units/mg protein
Poids mol.
280 kDa
Composition
Protein, ≥45% biuret
pl
~5.3
Solubilité
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Application
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Actions biochimiques/physiologiques
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Attention
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Forme physique
Lyophilized powder containing Tris buffer salts
Remarque sur l'analyse
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Daniel A S Kitagawa et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1370-1377 (2021-06-22)
Organophosphorus poisoning caused by some pesticides and nerve agents is a life-threating condition that must be swiftly addressed to avoid casualties. Despite the availability of medical countermeasures, the clinically available compounds lack a broad spectrum, are not effective towards all
Daniela Pereira et al.
Marine drugs, 19(1) (2020-12-31)
Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Joana R Almeida et al.
Biomolecules, 10(8) (2020-08-06)
The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series
Nagham Alatrash et al.
ChemMedChem, 12(13), 1055-1069 (2017-06-13)
Four mononuclear [(L-L)2 Ru(tatpp)]2+ and two dinuclear [(L-L)2 Ru(tatpp)Ru(L-L)2 ]4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22-tetraazatetrapyrido[3,2-a:2',3'-c:3'',2''-l:2''',3'''-n]pentacene (tatpp) ligand were synthesized, in which L-L is a chelating diamine ligand such as 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen), 3,4,7,8-tetramethyl-1,10-phenanthroline (Me4 phen) or 4,7-diphenyl-1,10-phenanthroline
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique