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Principaux documents

B8631

Sigma-Aldrich

Bile extract porcine

Synonyme(s) :

Bile acids, Hog Bile Powder

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About This Item

Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

Porcine

Niveau de qualité

Forme

powder

Technique(s)

microbiological culture: suitable

Application

Bile extract porcine has been used:
  • as a supplement in brain heart infusion (BHI) medium to culture Listeria monocytogenes LO28[1]
  • to investigate the survival of probiotic bacteria through the gastric-small intestine[2]
  • as a supplement in BHI mediato study the effect of oxygen on bile resistance in Listeria monocytogenes[3]

Actions biochimiques/physiologiques

Bile from bovine and ovine can be used in bacteriological research to investigate bile salt adaptation of bacteria such as Lactobacillus.

Qualité

Contains glycine and taurine conjugates of hyodeoxycholic acid and other bile salts.

Méthode d'analyse

Chemical derivatization and HPLC have been proven for separation and identification of complex mixtures of bile acids and bile acid conjugates.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Cristina Tudor et al.
Nutrients, 12(1) (2020-01-02)
Sea buckthorn oil, derived from the fruits of the shrub, also termed seaberry or sandthorn, is without doubt a strikingly rich source of carotenoids, in particular zeaxanthin and β-carotene. In the present study, sea buckthorn oil and an oil-in-water emulsion
Armando Hernández et al.
AMB Express, 9(1), 66-66 (2019-05-19)
This study was undertaken to investigate the impact of culture pH (4.5-6.5) and temperature (32-37 °C) on the stress resilience of Lactobacillus reuteri DSM 17938 during freeze-drying and post freeze-drying exposure to low pH (pH 2) and bile salts. Response-surface methodology
Rong Liang et al.
Journal of agricultural and food chemistry, 64(48), 9180-9188 (2016-12-10)
In order to improve the chemical stability and antioxidant activity of (-)-epigallocatechin gallate (EGCG) in the gastrointestinal tract, niosomes composed of Tween-60 and cholesterol were developed to encapsulate EGCG in this investigation. EGCG loaded niosomes with encapsulation efficiency around 76%
Diana Serra et al.
Nutrients, 12(12) (2020-12-03)
Dietary polyphenols are multi-target compounds that have been considered promising candidates in strategies for the mitigation of neurological diseases, acting particularly through reduction of microglia-driven neuroinflammation. In this study, an anthocyanin-rich extract obtained from Portuguese blueberries was subjected to a
Gabriele Rocchetti et al.
Antioxidants (Basel, Switzerland), 9(4) (2020-04-10)
In this work, different commercial extra-virgin olive oils (EVOO) were subjected to in vitro gastrointestinal digestion and the changes in bioactive compounds were evaluated by ultra-high-pressure liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry, using untargeted metabolomics. As expected, raw EVOO

Questions

1–5 of 5 Questions  
  1. What is the preparation method for B8631 and its solubility?

    1 answer
    1. The product B8631 should be considered a crude extract. In terms of solubility, quality control testing indicates that this product is tested for solubility in 80% ethanol at a concentration of 10 mg/mL, resulting in a slightly hazy to very hazy, very dark yellow solution. The color of the solution is within the acceptable range specified in the current Specification Sheet.

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  2. I would like to know if this product contains taurocholic acid and sodium taurodeoxycholate and how much of each. Thank you

    1 answer
    1. Taurocholic acid and sodium taurodeoxycholate are not tested on a lot to lot basis for this product. However, historical data reveals that typically this material contains approximately 4-7% taurodeoxycholic acid and 30-40% taurocholic acid.

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  3. What are the best solvents that can be used to solubilize this product?

    1 answer
    1. This product is soluble in 80% ethanol at 10mg/mL which yields a slightly hazy to very hazy solution. This material may also be dissolved in water at 50mg/mL. However, this will yield a turbid solution.

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  4. What is the content of cholesterol (it should mostly be free cholesterol) in B8381 and B8631?

    1 answer
    1. Neither B8381 nor B8631 have been evaluated for cholesterol content. The approximate amount of known components of both are listed below:
      8.0 - 9.5 % cholic acid
      0.9 - 1.1 % deoxycholic acid
      32 - 36 % conjugated cholic acid
      11 - 13 % conjugated deoxycholic acid

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  5. What is the proper temperature for storage?

    1 answer
    1. This product is stored at room temperature.

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