A0521

α-Amylase from human saliva

Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

• Synonyme(s) : 1,4-α-D-Glucan-glucanohydrolase
• Numéro CAS: 9000-90-2
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-565-6
• NACRES: NA.54
• MDL number: MFCD00081319
• Numéro CE : 3.2.1.1 (BRENDA, IUBMB)
• Specific activity: 1,000-3,000 units/mg protein

Propriétés

• type: Type IX-A
• Quality Segment: 200
• form: lyophilized powder
• specific activity: 1,000-3,000 units/mg protein
• composition: Protein, 5.0-15.0% biuret
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• UniProt accession no.: P0DUB6
• greener alternative category: Enabling,
• storage temp.: −20°C
• Gene Information: human ... AMY1A(276)

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.

Application

α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).

Biochem/physiol Actions

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Physical form

Lyophilized powder containing (NH₄)₂SO₄ and sodium citrate

Preparation Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• Hazard Classifications: Resp. Sens. 1
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable