A8419
Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat
IgG fraction of antiserum, buffered aqueous solution
Synonyme(s) :
Goat Anti-Human IgG (gamma-chain specific) HRP
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About This Item
Produits recommandés
Source biologique
goat
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
IgG fraction of antiserum
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Technique(s)
direct ELISA: 1:40,000
dot blot: 1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The specificity of Anti-Human IgG (γ-chain specific)-Peroxidase antibody is determined by immunoelectrophoresis and Ouchterlony Double Diffusion techniques. This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Spécificité
This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.
Immunogène
Human IgG
Application
Anti-Human IgG (γ-chain specific) Peroxidase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and western blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
IgM concentration in sera from patients with chronic CA syndrome was determined by ELISA using HRP-conjugated goat anti-human (mu chain specific) as the secondary antibody.
Actions biochimiques/physiologiques
Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.
Forme physique
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Notes préparatoires
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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