Unfortunately, the long term stability of the prepared solution has not been determined. However, prepared solutions are expected to be quite stable. Similar Sodium Carbonate/Bicarbonate 20X solutions are assigned a 2 year shelf life. Provided that solutions are stored refrigerated and not contaminated, a comparable shelf life is expected. Solutions should be observed for any changes in color and turbidity. Please see the link below to review the prepared 20X Concentrate product options:
https://www.sigmaaldrich.com/substance/sodiumbicarbonatesodiumcarbonateconcentrate1234598765
C3041
Tampon carbonate-bicarbonate
Carbonate-Bicarbonate Buffer
capsule
Synonyme(s) :
Solution tampon de carbonate
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About This Item
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Forme
capsule
Niveau de qualité
pH
9.3-9.9
Solubilité
water, high purity: 1 capsule/100 mL
Application(s)
diagnostic assay manufacturing
Catégories apparentées
Description générale
Les tampons bicarbonates ont généralement un pH d′environ 7,4. C′est le principal tampon pour l′intestin grêle.[1]
Application
Le tampon carbonate-bicarbonate a été utilisé pour suspendre des échantillons lyophilisés de plasma humain pour l′essai de biofilm sur microplaques et l′essai de biofilm dans une cellule de flux.[2] Il a également été utilisé comme tampon pour revêtir les microplaques avant les tests ELISA.[3][4]
Une fois préparé, ce tampon peut être utilisé dans des procédés de dépôt de protéines sur plaques multipuits ou tubes plastiques.
Reconstitution
Vider le contenu d′une gélose dans 100 ml d′eau désionisée et dissoudre. Le contenu d′une gélose produit 100 ml de 0,05 M de tampon carbonate-bicarbonate à pH 9,6 et 25 °C.
En option
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
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Protocoles
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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If I make a solution and there are reagents left, how long can it be stored? What should I do with the storage conditions?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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