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Merck
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Principaux documents

C3041

Sigma-Aldrich

Tampon carbonate-bicarbonate

Carbonate-Bicarbonate Buffer

capsule

Synonyme(s) :

Solution tampon de carbonate

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

capsule

Niveau de qualité

pH

9.3-9.9

Solubilité

water, high purity: 1 capsule/100 mL

Application(s)

diagnostic assay manufacturing

Description générale

Les tampons bicarbonates ont généralement un pH d′environ 7,4. C′est le principal tampon pour l′intestin grêle.[1]

Application

Le tampon carbonate-bicarbonate a été utilisé pour suspendre des échantillons lyophilisés de plasma humain pour l′essai de biofilm sur microplaques et l′essai de biofilm dans une cellule de flux.[2] Il a également été utilisé comme tampon pour revêtir les microplaques avant les tests ELISA.[3][4]
Une fois préparé, ce tampon peut être utilisé dans des procédés de dépôt de protéines sur plaques multipuits ou tubes plastiques.

Reconstitution

Vider le contenu d′une gélose dans 100 ml d′eau désionisée et dissoudre. Le contenu d′une gélose produit 100 ml de 0,05 M de tampon carbonate-bicarbonate à pH 9,6 et 25 °C.

En option

Réf. du produit
Description
Tarif

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Quantifying and comparing constitutive immunity across avian species
Millet S, et al.
Developmental and Comparative Immunology, 31(2), 188-201 (2007)
T J Caperna et al.
Domestic animal endocrinology, 60, 75-82 (2017-05-30)
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
Evolution of a physiological pH 6.8 bicarbonate buffer system: application to the dissolution testing of enteric coated products
Liu F, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 78(1), 151-157 (2011)
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
Yuki Noguchi et al.
Scientific reports, 10(1), 16268-16268 (2020-10-03)
In vivo pharmacokinetics (PK) studies using mice and monkeys are the main approaches for evaluating and predicting the PK of antibodies, and there is a strong demand for methods that do not require animal experiments. In this work, we focused

Protocoles

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Questions

1–3 of 3 Questions  
  1. If I make a solution and there are reagents left, how long can it be stored? What should I do with the storage conditions?

    1 answer
    1. Unfortunately, the long term stability of the prepared solution has not been determined. However, prepared solutions are expected to be quite stable. Similar Sodium Carbonate/Bicarbonate 20X solutions are assigned a 2 year shelf life. Provided that solutions are stored refrigerated and not contaminated, a comparable shelf life is expected. Solutions should be observed for any changes in color and turbidity. Please see the link below to review the prepared 20X Concentrate product options:
      https://www.sigmaaldrich.com/substance/sodiumbicarbonatesodiumcarbonateconcentrate1234598765

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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Reviews

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  1. Sanford, NC
    • Review 1
    • Votes 2
    5 out of 5 stars.

    Came as ordered, worked as expected

    The product was delivered on-time with helpful emails confirming my order and informing me of deliver times. The product worked as expected. Glad to be able to order the amounts that are needed and not have to order excess.

    Helpful?

  2. 1 Ratings-Only Review

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