The specific method used to test this material for protease activity is considered proprietary. However, it is based on the enzymatic cleavage of FITC-Casein. See the link below to review the FITC-Casein Enzymatic Assay to demonstrate the principle of this method.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/850/c3777enz.pdf
A3059
Albumine de sérum bovin
heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Synonyme(s) :
Albumine bovine, Albumine, sérum bovin, BSA, ASB
About This Item
Produits recommandés
Source biologique
bovine
Niveau de qualité
Essai
≥98%
Forme
lyophilized powder
Poids mol.
~66 kDa
Produit purifié par
heat shock fractionation
Conditionnement
poly bottle of
Origine
USA origin
Technique(s)
ELISA: suitable
western blot: suitable
Impuretés
essentially globulin free
protease, none detected
Perte
≤5%
pH
7
Solubilité
water: soluble (40 mg/ml)
Numéro d'accès UniProt
Activité étrangère
BT Virus, none detected
VSV Virus, none detected
Température de stockage
2-8°C
Informations sur le gène
bovine ... ALB(280717)
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Description générale
- Quantification des protéines (comme étalon)
- Immunochimie (comme agent de blocage)
- Culture cellulaire (comme nutriment)
La très faible teneur ou l'absence de protéases de cette BSA est spécifiquement évaluée et testée. Cette BSA dépourvue de protéases peut notamment être utilisée dans les applications suivantes :
- Dosages enzymatiques
- Tests utilisant des protéines
- Techniques sensibles aux protéases, dont :
b) Hybridation d'acides nucléiques
c) Dosages radio-immunologiques (RIA)
Application
Actions biochimiques/physiologiques
Caractéristiques et avantages
- Fractionnement par choc thermique
- Absence de protéases
- Quasi-absence de globulines
Notes préparatoires
Souvent commandé avec ce produit
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Certificats d'analyse (COA)
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Contenu apparenté
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
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Which method do you use to determine the protease concentration?
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What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)? Can it be considered fatty acid free?
1 answer-
The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?
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We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.
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Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?
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We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?
1 answer-
We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background. The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present".
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases?
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A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.
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