SCR028
Mesenchymal Stem Cell Osteogenesis Kit
The Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining.
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352207
eCl@ss :
32161000
Nomenclature NACRES :
NA.81
Produits recommandés
Niveau de qualité
Fabricant/nom de marque
Chemicon®
Technique(s)
cell culture | stem cell: suitable
Entrée
sample type mesenchymal stem cell(s)
Méthode de détection
colorimetric
Conditions d'expédition
wet ice
Description générale
Bone undergoes a continual remodeling process that requires the coordinated activity of two types of cells. Osteoclasts break down the bone matrix while osteoblasts deposits collagen, calcium, and phosphorous and other minerals to form new bone. The balance between the activity of osteoclasts and osteoblasts determine the mass and density of the bone. Many diseases of bone including osteoporosis, a common age-related phenomenon in post-menopausal women in which the bone mass has been greatly reduced, and osteogenesis imperfecta, also known as brittle-bone disease, are likely caused by the misregulation of osteoblasts and osteoclasts. Understanding the molecular mechanisms that underlie osteogenesis, the process by which new bone is formed is thus of critical importance.
Stem cell technology, particularly embryonic stem cells and/or mesenchymal stem cells, offer attractive sources of osteoblasts for tissue culture studies and for the biochemical dissection of the earliest steps involved in osteoblast cell determination. Mesenchymal stem cells are multipotent progenitor cells that have the capacity to differentiate into several mesenchymal cell lineages, including bone, cartilage and fat.
CHEMICON®′s Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining. Reagents in the kit include two ECM coating molecules (collagen Type I and vitronectin) that have been shown to promote osteogenic differentiation of mesenchymal stem cells (Salasznyk, 2004) along with inducing reagents, dexamethasone, ascorbic acid 2-phosphate and b-glycerophosphate. Also included is Alizarin Red Solution, a staining solution that is used to detect the presence of calcium in bone.
Using CHEMICON′s Mesenchymal Stem Cell Osteogenesis Kit, we typically obtain >50% mature osteocytes from rat bone marrow derived mesenchymal stem cells. Efficiency of osteogenic differentiation may vary, depending upon the quality of the mesenchymal stem cells and if variations to the protocol are introduced.
For Research Use Only; not for use in diagnostic procedure.
Stem cell technology, particularly embryonic stem cells and/or mesenchymal stem cells, offer attractive sources of osteoblasts for tissue culture studies and for the biochemical dissection of the earliest steps involved in osteoblast cell determination. Mesenchymal stem cells are multipotent progenitor cells that have the capacity to differentiate into several mesenchymal cell lineages, including bone, cartilage and fat.
CHEMICON®′s Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining. Reagents in the kit include two ECM coating molecules (collagen Type I and vitronectin) that have been shown to promote osteogenic differentiation of mesenchymal stem cells (Salasznyk, 2004) along with inducing reagents, dexamethasone, ascorbic acid 2-phosphate and b-glycerophosphate. Also included is Alizarin Red Solution, a staining solution that is used to detect the presence of calcium in bone.
Using CHEMICON′s Mesenchymal Stem Cell Osteogenesis Kit, we typically obtain >50% mature osteocytes from rat bone marrow derived mesenchymal stem cells. Efficiency of osteogenic differentiation may vary, depending upon the quality of the mesenchymal stem cells and if variations to the protocol are introduced.
For Research Use Only; not for use in diagnostic procedure.
Application
Research Category
Stem Cell Research
Stem Cell Research
The Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining.
Composants
Dexamethasone Solution: (Catalog No. 90357) One vial containing 100 μL of 10 mM dexamethasone in ethanol. Store at -20°C.
Ascorbic Acid 2-Phosphate Solution: (Catalog No. 2004011) One vial containing 500 μL of 100 mM ascorbic acid 2-phosphate in water. Store at -20°C.
Glycerol 2- Phosphate Solution: (Catalog No. 2004010) Three vials containing 1 mL of 1 M glycerol 2-phosphate in water. Store at -20°C.
Collagen, Type I: (Catalog No. 2004013) One vial containing 150 μg collagen Type I. Store at -20°C.
Vitronectin: (Catalog No. 2004012) One vial containing 150 μg vitronectin. Store at -20°C.
Alizarin Red Solution: (Catalog No. 2003999) One bottle containing 50 mL Alizarin Red Solution. Store at 2° to 8°C.
Ascorbic Acid 2-Phosphate Solution: (Catalog No. 2004011) One vial containing 500 μL of 100 mM ascorbic acid 2-phosphate in water. Store at -20°C.
Glycerol 2- Phosphate Solution: (Catalog No. 2004010) Three vials containing 1 mL of 1 M glycerol 2-phosphate in water. Store at -20°C.
Collagen, Type I: (Catalog No. 2004013) One vial containing 150 μg collagen Type I. Store at -20°C.
Vitronectin: (Catalog No. 2004012) One vial containing 150 μg vitronectin. Store at -20°C.
Alizarin Red Solution: (Catalog No. 2003999) One bottle containing 50 mL Alizarin Red Solution. Store at 2° to 8°C.
Stockage et stabilité
Note: Kit components require two different storage temperatures.
Dexamethasone Solution, Ascorbic Acid 2-Phosphate Solution, Glycerol 2- Phosphate Solution, Collagen Type I, and Vitronectin should be stored at -20ºC. Alizarin Red Solution should be stored at 2° to 8°C.
Precautions
· Dexamethasone is an irritant and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions.
Please refer to the Material Safety Data Sheet at www.chemicon.com for further precautions.
Dexamethasone Solution, Ascorbic Acid 2-Phosphate Solution, Glycerol 2- Phosphate Solution, Collagen Type I, and Vitronectin should be stored at -20ºC. Alizarin Red Solution should be stored at 2° to 8°C.
Precautions
· Dexamethasone is an irritant and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions.
Please refer to the Material Safety Data Sheet at www.chemicon.com for further precautions.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2 - Repr. 1B - STOT SE 2
Organes cibles
Eyes,Central nervous system
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
48.2 °F
Point d'éclair (°C)
9 °C
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Tapas R Nayak et al.
ACS nano, 5(6), 4670-4678 (2011-05-03)
Current tissue engineering approaches combine different scaffold materials with living cells to provide biological substitutes that can repair and eventually improve tissue functions. Both natural and synthetic materials have been fabricated for transplantation of stem cells and their specific differentiation
Pooi Ling Mok et al.
International journal of molecular sciences, 18(2) (2017-02-18)
Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles
Yin Yin Ooi et al.
World journal of stem cells, 5(1), 34-42 (2013-01-31)
To assess the capacity to isolate and expand mesenchymal stem cells (MSC) from bone marrow of CBA/Ca, ICR and Balb/c mice. Bone marrow of tibia and femur were flushed, cultured and maintained in supplemented Dulbecco's modified Eagle's medium. MSC immunophenotype
Jianying Zhang et al.
BMC musculoskeletal disorders, 11, 10-10 (2010-01-20)
Tendons are traditionally thought to consist of tenocytes only, the resident cells of tendons; however, a recent study has demonstrated that human and mouse tendons also contain stem cells, referred to as tendon stem/progenitor cells (TSCs). However, the differential properties
Zul'atfi Rahmat et al.
Stem cell research & therapy, 4(1), 12-12 (2013-01-30)
Mesenchymal stem cells (MSCs) are immunosuppressive, but we lack an understanding of how these adult stem cells are in turn affected by immune cells and the surrounding tissue environment. As MSCs have stromal functions and exhibit great plasticity, the influence
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique