MAC111

Anti-GST•Tag fusion protein Antibody

clone 8-326.6.1, from mouse

• Synonyme(s) : Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 8-326.6.1, monoclonal
• Réactivité de l'espèce (prédite par homologie): all
• Technique(s): ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès UniProt: P08515
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

GST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress.

Immunogène

Gluththione-S-transferase (GST) domain from Schistosoma Japonicum

Application

Immunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP.

ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.

Qualité

Evaluated by Western Blot in Posi-Tag Epitope Tag Control.

Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control.

Description de la cible

~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Posi-Tag Epitope Tag Control

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable