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A propos de cet article
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, ELISA (i), WB
Citations:
192
Service technique
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rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
technique(s)
dot blot: suitable, indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. The soluble GST is encoded by eight different genes in humans namely, α, μ, θ, π, ζ, σ, κ and ω on chromosome 6, 1, 22, 11, 14, 4, not known and 10 respectively.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.
Immunogen
recombinant GST from Schistosoma japonicum expressed in E. coli.
Application
Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit has been used in immunoblotting.
Biochem/physiol Actions
Glutathione-S-transferase (GST) catalyzes the detoxication of electrophiles by glutathione conjugation. This enzyme provides protection against oxidative stress and toxic foreign chemicals. Polymorphism in GST gene is associated with the development of asthma and cutaneous basal cell carcinoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
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Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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