This product reconstituted to 0.365 g/L yields a molarity of approximately 0.0025 mol/L (2.5 mM). Please see the link below to review the full formulation:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/822/486/d0547pis-ms.pdf
D8900
Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham
With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
DME/F-12, mélange 1/1
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| À vous/Référence | Disponibilité | Prix |
|---|
A propos de cet article
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderQuality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:
- as a component of the culture medium for endometrial explants in vitro culture
- to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
- to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
1 of 1
Cet article | |||
|---|---|---|---|
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components sodium pyruvate: 0.055 g/L | components NaHCO3: no | components NaHCO3: no | components glucose: 3.15 g/L |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
Still not finding the right product?
Explore all of our products under Milieu de Eagle modifié par Dulbecco (DMEM)/Mélange nutritif F-12 de Ham
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
-
What is the molarity of L-glutamine when reconstituted?
1 answer-
Helpful?
-
-
What is the molarity of glucose when reconstituted with water?
1 answer-
Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).
Helpful?
-