ABN1469

Anti-Alpha-2-HS-Glycoprotein

from rabbit, purified by affinity chromatography

• Synonyme(s) : Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2 Heremans-Schmid glycoprotein, AHGS
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001613.2
• Numéro d'accès UniProt: P02765
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AHSG(197)

Description

Description générale

Alpha-2-HS-glycoprotein (UniProt: P02765; also known as Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A, Alpha-2 Heremans-Schmid glycoprotein, AHSG) is encoded by the AHSG (also known as FETUA, PRO2743) gene (Gene ID: 197) in human. Alpha-2-HS-glycoprotein is a serum protein of the cystatin superfamily that is synthesized by hepatocytes and is considered as a negative acute-phase reactant. It is highly expressed in various fetal tissues, but in adults it is mainly expressed in the liver. It is synthesized with a signal peptide (aa 1-18) and a connective propeptide (aa 301-340) and is subsequently cleaved into 2 chains known as Alpha-2-HS-glycoprotein chain A and Alpha-2-HS-glycoprotein chain B. The mature product is involved in multiple normal biological and pathological functions. It promotes possesses opsonic properties and is shown to be involved in endocytosis, brain development, bone resorption, regulation of insulin activity and hepatocyte growth factor (HGF) activity. Alpha-2-HS-glycoprotein also serves as a carrier protein and serves as a carrier for otherwise insoluble calcium phosphate and can act as a potent inhibitor of pathological vascular calcification. Higher levels of Alpha-2-HS-glycoprotein have been reported in gestational and type II diabetes. It is also present at higher levels in patients with endometriosis. Lower levels of Alpha-2-HS-glycoprotein are present in cancer subjects. (Ref.: Kalabay, L., et al. (2002). Eur. J. Endocrinol. 147(2); 243-248; Sindhu, S., et al. (2016). BMC Immunol. 17; 33).

Spécificité

This rabbit polyclonal antibody specifically detects human Alpha-2-HS-glycoprotein. It targets an epitope with in the N-terminal half.

Immunogène

Epitope: N-terminus

GST/His-tagged recombinant fragment corresponding to 146 amino acids from human Alpha-2-HS-glycoprotein.

Application

Anti-Alpha-2-HS-Glycoprotein Antibody, Cat. No. ABN1469, is a highly specific rabbit polyclonal antibody that targets Alpha-2-HS-Glycoprotein and has been tested for use Immunohistochemistry and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Alpha-2-HS-Glycoprotein in human retina tissue lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Alpha-2-HS-Glycoprotein in human liver and human bone marrow tissue sections.

Qualité

Evaluated by Western Blotting in human serum.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Alpha-2-HS-Glycoprotein in human serum.

Description de la cible

~55 kDa observed; 39.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.