F3385

Fetuin from fetal calf serum

lyophilized powder, BioReagent, suitable for cell culture

• Synonyme(s) : Fetuin from fetal calf serum
• Numéro CAS: 9014-81-7
• UNSPSC Code: 12352202
• EC Number: 232-762-7
• NACRES: NA.75
• MDL number: MFCD00131055
• Form: lyophilized powder
• Mol wt: 48.4 kDa

Propriétés

• product line: BioReagent
• Quality Segment: 200
• form: lyophilized powder
• mol wt: 48.4 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.3% free N-acetylneuraminic acid
• solubility: H₂O: 1 mg/mL
• storage temp.: 2-8°C

Description

General description

Fetuin from fetal bovine serum is a plasma glycoprotein. It is a 64 kDa glycoprotein, which is produced by the liver and adipose tissue.
A glycoprotein derived from FBS that is used as Fetuin has been used at a concentration of 500 mg/ml to supplement serum free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells.

Application

Fetuin from fetal bovine serum has been used:

• as a substrate to determine neuraminidase activity
• to validate the specificity of sialidases
• to stabilize m-calpain

Biochem/physiol Actions

Fetuin acts as an inhibitor of insulin receptor tyrosine kinase. It modulates bone remodeling and calcium metabolism. Fetuin regulates vascular calcification, insulin resistance, protease activity control, keratinocytes migration and breast tumor cell proliferative signaling. It functions as a biomarker for neurodegenerative diseases. Fetuin exhibits anti-inflammatory property.

Preparation Note

Prepared by ammonium sulfate fractionation of fetal bovine serum by the method of Pederson, K.O., J. Phys. and Colloid Chem., 51, 164 (1947).

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)