07-1466
Anti-Cdc42 Antibody
from rabbit
Synonyme(s) :
G25K, GTP-binding Protein
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human, rat
Technique(s)
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CDC42(998)
Description générale
cdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42.
Spécificité
Recognizes Cdc42, does not cross react with Rac1. Cdc42 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Cdc42 induces the formation of filopodia. Cdc42 can activate Rac (consequently, filopodia are intimately associated with lamellipodia.
Immunogène
Synthetic peptide corresponding to amino acids 130-141 of human Cdc42
(AKNKQKPITPET-C).
(AKNKQKPITPET-C).
Application
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-Cdc42 Antibody is validated for use in WB for the detection of Cdc42.
Western Blot Analysis: Rat brain with previous lots of rabbit anti-Cdc42 (cat. # 07-1466) and rabbit anti-Rac1 (cat. # 07-14664).
The molecular weights of Rac1 and Cdc42 are ~21 kDa.
Optimal working dilutions must be determined by end user.
The molecular weights of Rac1 and Cdc42 are ~21 kDa.
Optimal working dilutions must be determined by end user.
Qualité
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.
Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.
Description de la cible
21 kDa
Liaison
Replaces: AB4201
Forme physique
Affinity Purified
Format: Purified
Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.
Stockage et stabilité
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Remarque sur l'analyse
Control
Rat Brain Lysate
Rat Brain Lysate
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Edward O Kwarteng et al.
Journal of leukocyte biology, 103(3), 381-393 (2018-01-19)
The switch from fetal to adult hematopoietic stem/progenitor cells (HSPCs) is associated with profound changes in several genetic programs. Although HSPC ageing corresponds to alterations in Wnt signaling, relatively little is known about the relative roles of different Wnt signaling
Shiri Gur-Cohen et al.
Nature medicine, 21(11), 1307-1317 (2015-10-13)
Retention of long-term repopulating hematopoietic stem cells (LT-HSCs) in the bone marrow is essential for hematopoiesis and for protection from myelotoxic injury. We report that signaling cascades that are traditionally viewed as coagulation related also control retention of endothelial protein
Amanda Amoah et al.
Haematologica (2021-01-15)
In this study, we characterize age-related phenotypes of human hematopoietic stem cells (HSCs). We report increased frequencies of HSC, HPC and lineage negative cells in the elderly but a decreased frequency of multi-lymphoid progenitors. Aged human HSCs further exhibited a
Elodie Grockowiak et al.
Nature cancer, 4(8), 1193-1209 (2023-08-08)
Aging facilitates the expansion of hematopoietic stem cells (HSCs) carrying clonal hematopoiesis-related somatic mutations and the development of myeloid malignancies, such as myeloproliferative neoplasms (MPNs). While cooperating mutations can cause transformation, it is unclear whether distinct bone marrow (BM) HSC-niches
Yang Liu et al.
Developmental biology, 383(2), 239-252 (2013-09-24)
Neural crest cells (NCCs) are physically responsible for craniofacial skeleton formation, pharyngeal arch artery remodeling and cardiac outflow tract septation during vertebrate development. Cdc42 (cell division cycle 42) is a Rho family small GTP-binding protein that works as a molecular
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