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Key Documents

219215

Sigma-Aldrich

Leucocrystal Violet

Synonyme(s) :

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Formule linéaire :
CH[C6H4N(CH3)2]3
Numéro CAS:
Poids moléculaire :
373.53
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.47

Forme

powder

Pf

175-177 °C (lit.)

λmax

260 nm

ε (coefficient d'extinction)

≥700 at 257-263 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

Clé InChI

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Actions biochimiques/physiologiques

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Attention

Photo-ionization can cause color variations

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Environment

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

J E Roybal et al.
Journal of chromatography, 467(1), 259-266 (1989-04-21)
High-performance liquid chromatographic conditions are reported for the electrochemical detection (ED) of Gentian Violet, its demethylated metabolites, Leucogentian Violet and Methylene Blue. Gentian Violet, its demethylated metabolites and Leucogentian Violet were separated within 14 min on a cyano column eluted
R T Wilson et al.
Journal of AOAC International, 77(5), 1137-1142 (1994-09-01)
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated
Xueli Wu et al.
Journal of chromatography. A, 1172(2), 121-126 (2007-10-24)
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and
J E Roybal et al.
Journal - Association of Official Analytical Chemists, 73(6), 940-946 (1990-11-01)
A method is presented for determination of residues of gentian violet (GV), its demethylated metabolites (pentamethyl and tetramethyl), and leucogentian violet (LGV) in chicken tissue. The analytes are extracted from tissue with acetonitrile/buffer and partitioned into methylene chloride. Polar lipids
J J McDonald et al.
Drug metabolism and disposition: the biological fate of chemicals, 12(3), 330-336 (1984-05-01)
Cellular suspensions of microflora isolated from human feces and from intestinal contents of rat and chicken transformed the triphenylmethane dye gentian violet under anaerobic conditions. Pure cultures of nine genera of strict and facultative anaerobes were also active. All pure

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