B3133

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, ≥99% (TLC), powder, suitable for substrate for trypsin

• Synonym(s): L-BAPA, L-BAPNA, BANI
• Empirical Formula (Hill Notation): C₁₉H₂₂N₆O₄ · HCl
• CAS Number: 21653-40-7
• Molecular Weight: 434.88
• Beilstein: 4081878
• EC Number: 244-505-6
• MDL number: MFCD00063682
• UNSPSC Code: 12352204
• PubChem Substance ID: 24891685
• NACRES: NA.32

Properties

• product name: N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride, ≥99% (TLC), suitable for substrate for trypsin
• Assay: ≥99% (TLC)
• form: powder
• solubility: acetone: water (1:1): 50 mg/mL
• suitability: suitable for substrate for trypsin
• storage temp.: −20°C
• SMILES string: Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1
• InChI key: DEOKFPFLXFNAON-NTISSMGPSA-N

Description

General description

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a substrate for trypsin.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as a substrate:

• for chymotrypsin and trypsin activity assay in enzymatic extract (EE) of M. tenellum and pancreatic tissue homogenates
• in trypsin and papain standard curve generation
• in serine protease and fibrinogenolytic assays of eupolytin1 protein

Linkage

Similar to B3279, but produced for Sigma.

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves