P4557

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

• Synonym(s): Tris-equilabrated Phenol
• Empirical Formula (Hill Notation): C₆H₆O
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• Beilstein: 6428463
• EC Number: 203-632-7
• MDL number: MFCD00002143
• UNSPSC Code: 12352104
• PubChem Substance ID: 57654529
• NACRES: NA.52

Properties

• grade: for molecular biology
• product line: BioReagent
• Assay: ≥99.0% (GC)
• form: liquid
• pH: 7.7-8.1 (25 °C)
• storage temp.: 2-8°C
• SMILES string: Oc1ccccc1
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• Gene Information: human ... GABRA1(2554)

Description

General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

• as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
• for extraction of DNA from Arabidopsis thaliana Rosette leaves
• in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
• as a component of extraction buffer to extract RNA from fruit pulp
• for the extraction of protein from roots
• for the isolation of fecal DNA
• for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

Components

• 1 bottle Tris-equilabrated Phenol (product number P4557)
• Equilibration Buffer (product B5658)

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H311 + H331,H314,H341,H373,H411
• Precautionary Statements: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Target Organs: Nervous system,Kidney,Liver,Skin
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves