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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Synonym(s):

phenol chloroform RNA extraction

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100 ML
617,00 kr.
400 ML
1.880,00 kr.

617,00 kr.

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100 ML
617,00 kr.
400 ML
1.880,00 kr.

About This Item

UNSPSC Code:
12190000
NACRES:
NA.52
grade:
for molecular biology
Assay:
≥99.0%
application(s):
life science and biopharma

617,00 kr.

Please contact Customer Service for Availability

grade

for molecular biology

Quality Level

200

Assay

≥99.0%

form

liquid

pH

4.3-4.7

application(s)

life science and biopharma

storage temp.

2-8°C

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.[1]

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.[2][3][4]
  • to isolate RNA from Arabidopsis siliques.[5]
  • to extract proteins and DNA from polysomal and non-polysomal fractions.[6]
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.[7]

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Target Organs

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

175.0 °F - closed cup

Flash Point(C)

79.44 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW7584
MKCV5362
MKCV5361
MKCT1646
MKCT0359

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Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
C Pistore et al.
Oncogene, 36(40), 5551-5566 (2017-06-06)
Widespread genome hypo-methylation and promoter hyper-methylation of epithelium-specific genes are hallmarks of stable epithelial-to-mesenchymal transition (EMT), which in prostate cancer (PCa) correlates with castration resistance, cancer stem cells generation, chemoresistance and worst prognosis. Exploiting our consolidated 'ex-vivo' system, we show
Exploring translation regulation by global analysis of ribosomal association
Melamed D, et al.
Methods in Enzymology, 48(3), 301-305 (2009)
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Questions

  1. Hello, Your product is fractionated into two phases. Should I use lower phase for RNA extraction? Thank you

    1 answer

    1. Product P1944 - Phenol:Chloroform: Iso-amyl alcohol (125:24:1) is acidic. As such, the RNA isolates in an acidic pH and will partition the genomic DNA into the organic phase leaving only the RNA in the top aqueous phase.

      Helpful?

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