HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
Materialien
Standard
Produkt-Nr.
Beschreibung
Preisangaben
Trypsin-Inhibitor aus Glycine max (soybean)
solution, sterile-filtered, suitable for, suitable for cell cultureTrypsin-Inhibitor aus Hühnereiweiss
Type II-O, Partially purified ovomucoid, containing ovoinhibitorTrypsin Inhibitor from Glycine max (soybean)
BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)CONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase
[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate
0.5 mL/min
detector
UV, 280 nm
injection
10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
Beschreibung
Hinweis zur Analyse
The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.
Rechtliche Hinweise
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany