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814475

TOYOPEARL® AF-Chelate-650M Bulk Media

phase IDA, iminodiacetic acid, bottle of 25 mL, 65 μm particle size

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About This Item

UNSPSC-Code:
41115700

Beschreibung

AF-Chelate-650M

Produktlinie

TOYOPEARL®

Form

liquid

Verpackung

bottle of 25 mL

Hersteller/Markenname

Tosoh TOYOPEARL® AF, 14475

Kennzeichnungsgrad

25-45 μeq/mL ligand density

Parameter

3 bar max. pressure

Methode(n)

HPLC: suitable

Matrix

hydroxylated methacrylic polymer

Aktive Matrixgruppe

IDA phase, iminodiacetic acid

Partikelgröße

65 μm

Betriebs-pH-Wert

2-12

Trenntechnik

affinity

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.

Anwendung

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Spezifikationen

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physikalische Form

Shipped in 20% (v/v) ethanol.

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Rechtliche Hinweise

Toyopearl is a registered trademark of Tosoh Corporation

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Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Gefahreneinstufungen

Flam. Liq. 3

WGK

WGK 1

Flammpunkt (°F)

103.1 °F

Flammpunkt (°C)

39.5 °C

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S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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