Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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53574-U
Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns
L × I.D. 3 cm × 3 mm, HPLC Column
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About This Item
UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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Produktbezeichnung
Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 3 cm × 3 mm
Materialien
stainless steel column
Agentur
suitable for USP L43
Produktlinie
Ascentis®
Leistungsmerkmale
endcapped
Hersteller/Markenname
Ascentis®
Verpackung
1 ea of
Parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
Methode(n)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × ID
3 cm × 3 mm
Oberflächenbereich
135 m2/g
Verunreinigungen
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
Aktive Matrixgruppe
PFP (pentafluorophenyl) phase
Partikelgröße
2.7 μm
Porengröße
90 Å pore size
Betriebs-pH-Wert
2-8
Anwendung(en)
food and beverages
Trenntechnik
reversed phase
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Allgemeine Beschreibung
The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Anwendung
Erforderlich, aber nicht bereitgestellt
Produkt-Nr.
Beschreibung
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Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Artikel
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
Protokolle
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
Chromatograms
application for HPLCapplication for HPLC-
Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 Antwort-
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Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 Antwort-
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 Antwort-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
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Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?
1 Antwort-
Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the pressure rating for Ascentis® Express F5 HPLC Columns?
1 Antwort-
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
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Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
1 Antwort-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Are guard columns available for Ascentis® Express F5 3.0 mm ID HPLC Columns?
1 Antwort-
Yes, we have guard columns available for product Ascentis® Express F5 3.0 mm ID HPLC Columns. Product 53500-U is the Ascentis® Express Guard Holder and Product 53597-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5 mm × I.D. 3.0 mm .
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 Antwort-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Can Ascentis® Express F5 HPLC columns be used for LC-MS?
1 Antwort-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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