The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
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About This Item
UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × ID:
5 cm × 4.6 mm
Oberflächenbereich:
135 m2/g
Partikelgröße:
2.7 μm
Aktive Matrixgruppe:
phenylhexyl phase
Porengröße:
90 Å
Matrix:
Fused-Core particle platform
superficially porous particle
superficially porous particle
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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Produktbezeichnung
Ascentis® Express Phenyl-Hexyl, 2,7 μm HPLC-Säule, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm
Materialien
stainless steel column
Qualitätsniveau
Agentur
suitable for USP L11
Produktlinie
Ascentis®
Leistungsmerkmale
endcapped
Hersteller/Markenname
Ascentis®
Verpackung
1 ea of
Parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
Methode(n)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × ID
5 cm × 4.6 mm
Oberflächenbereich
135 m2/g
Verunreinigungen
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
Aktive Matrixgruppe
phenylhexyl phase
Partikelgröße
2.7 μm
Porengröße
90 Å
pH-Bereich
2-9
Anwendung(en)
food and beverages
Trenntechnik
reversed phase
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Verwandte Kategorien
Allgemeine Beschreibung
Die Phenyl-Hexyl-Phase weist durch die Wechselwirkung zwischen der gelösten Substanz und dem aromatischen Ring und seinen delokalisierten Elektronen eine einzigartige Selektivität auf. Aufgrund dieser einzigartigen Aromatizität ist sie komplementär (orthogonal) zur C18- und RP-Amid-Phase. Des Weiteren neigt die Phenyl-Hexyl-Phase zu einer guten Formselektivität, die vermutlich aus der Mehrfachwechselwirkung zwischen der gelösten Substanz und dem planaren Ringsystem resultiert. Bei gelösten Stoffen mit aromatischen elektronenziehenden Gruppen (Fluor, Nitro usw.) oder mit einem delokalisierten heterozyklischen Ringsystem, wie bei Benzodiazepin-Verbindungen, wird häufig eine höhere Retention und Selektivität beobachtet.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Erforderlich, aber nicht bereitgestellt
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Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Artikel
Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.
Chromatograms
application for HPLCapplication for HPLC-
How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 Antwort-
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Can I use Ascentis Express on a UHPLC system?
1 Antwort-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?
1 Antwort-
The phenyl-hexyl phase is 100% aqueous compatible.
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What is the pressure rating for Ascentis® Express Phenyl-Hexyl HPLC Columns?
1 Antwort-
This Ascentis Express column is stable to operating pressures up to 600 bar (9000 psi).
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 Antwort-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Can I use Ascentis Express on any type of HPLC system?
1 Antwort-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 Antwort-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 Antwort-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 Antwort-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)
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