T4661

Trizma^® Base

≥99.9% (titration), crystalline

• Synonym(e): 2-Amino-2-(hydroxymethyl)-1,3-propandiol, THAM, Tris Base, Tris(hydroxymethyl)aminomethan, Trometamol
• Lineare Formel: NH₂C(CH₂OH)₃
• CAS-Nummer: 77-86-1
• Molekulargewicht: 121.14
• Beilstein: 741883
• EG-Nummer: 201-064-4
• MDL-Nummer: MFCD00004679
• UNSPSC-Code: 12352104
• PubChem Substanz-ID: 24900061
• NACRES: NA.25

Eigenschaften

• Beschreibung: aminopeptidase substrate
• Qualitätsniveau: 400
• Assay: ≥99.9% (titration)
• Form: crystalline
• Verlust: ≤5% (loss on drying, 110 °C)
• Farbe: white
• pH-Wert: 10.0-11.5
• Nützlicher pH-Bereich: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp (Schmelzpunkt): 167-172 °C (lit.)
• Löslichkeit: water: soluble (400 g plus 600 mL of water)
• λ: (290nm < 0.05); in 5 mM Tris; DNA (40% (w/w))
• Anwendung(en): diagnostic assay manufacturing
• SMILES String: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChIKey: LENZDBCJOHFCAS-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Anwendung

Tris base has been used:

• As a component of lysis buffer for cell disruption
• In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Verpackung

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Hinweis zur Analyse

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Sonstige Hinweise

Die pH-Werte aller Puffer sind temperatur- und konzentrationsabhängig. Bei Tris-Puffern steigt der pH-Wert um etwa 0,03 Einheiten pro °C Temperaturabfall und fällt 0,03-0,05 Einheiten pro Zehnfachverdünnung.
Verwenden Sie für präzise Anwendungen ein sorgfältig kalibriertes pH-Messgerät mit einer Glas/Kalomel-Kombielektrode.

Rechtliche Hinweise

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves