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T1763

Sigma-Aldrich

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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92,40 €
50 UNITS
161,00 €

92,40 €


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25 UNITS
92,40 €
50 UNITS
161,00 €

About This Item

EC-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
eCl@ss:
42020142
NACRES:
NA.54

92,40 €


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Biologische Quelle

bovine pancreas (trypsin)

Form

buffered aqueous suspension

Konzentration

≥15 units/mL (packed gel)

Kennzeichnungsgrad

≥15 units per mL packed gel

Matrix

cross-linked beaded agarose

Versandbedingung

wet ice

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.[1]

Anwendung

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. [2] Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. [3]
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins[4][5] and gliadin[5] to generate peptides.

Sonstige Hinweise

Insolubilized

Einheitendefinition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Physikalische Form

Suspension in approx. 10 mM acetic acid, pH 3.2

Piktogramme

Exclamation mark

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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S C Bracken et al.
Alimentary pharmacology & therapeutics, 23(9), 1307-1314 (2006-04-25)
In coeliac disease, wheat, barley and rye are traditionally excluded in the gluten-free diet. However, few studies have examined the small intestinal immune response to barley and rye. To investigate the immunogenicity of barley and rye prolamins (hordein and secalin
Amit Tripathi et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(39), 16799-16804 (2009-10-07)
Increased intestinal permeability (IP) has emerged recently as a common underlying mechanism in the pathogenesis of allergic, inflammatory, and autoimmune diseases. The characterization of zonulin, the only physiological mediator known to regulate IP reversibly, has remained elusive. Through proteomic analysis
Vanessa A Gutzeit et al.
Cell chemical biology, 28(11), 1648-1663 (2021-03-19)
Despite the power of photopharmacology for interrogating signaling proteins, many photopharmacological systems are limited by their efficiency, speed, or spectral properties. Here, we screen a library of azobenzene photoswitches and identify a urea-substituted "azobenzene-400" core that offers bistable switching between
Raquel Olías et al.
Food research international (Ottawa, Ont.), 169, 112825-112825 (2023-05-31)
The presence of so-called anti-nutritional factors can reduce the bioavailability of nutrients following consumption of seeds which are otherwise an excellent source of proteins, carbohydrates and micronutrients. Among the proteins associated with negative effects on quality in pea (Pisum sativum
R M Blanco et al.
Enzyme and microbial technology, 13(7), 573-583 (1991-07-01)
By using very active and very stable trypsin agarose derivatives, we have optimized the design of the synthesis of a model dipeptide, benzoylarginine leucinamide, by two different strategies: (i) kinetically controlled synthesis (KCS), by using benzoyl arginine ethyl ester and

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