SRP3330

IL-6 from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): BSF-2, HPGF, IFN-?2, B cell differentiation factor (BCDF)
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: 5x10^7 units/mg ED₅₀
• Mol-Gew.: 21.7 kDa
• Verpackung: pkg of 10 μg
• Verunreinigungen: endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P08505
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... IL6(16193)

Beschreibung

Allgemeine Beschreibung

Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 188 amino acid residues.

Anwendung

IL-6 from mouse has been used to study IL-6-mediated inhibition of mouse preadipocyte differentiation.[1]

Biochem./physiol. Wirkung

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R and gp130.[2]

Physikalische Form

Lyophilized from 0.1% TFA

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable