SAB4200255

Anti-PP2A subunit B isoform B56-δ antibody, Mouse monoclonal

clone H5D12, purified from hybridoma cell culture

• Synonym(e): Anti-PP2A B subunit isoform PR61-δ, Anti-PP2A B subunit isoform R5-δ, Anti-PPP2R5D, Anti-Protein phosphatase 2, regulatory subunit B′,δ, Anti-Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, δ isoform
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: H5D12, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~72 kDa
• Speziesreaktivität: human, rat
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS or rat Rat2 cells.
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q14738
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PPP2R5D(5528)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-PP2A subunit B isoform B56-δ (mouse IgG1 isotype) is derived from the hybridoma H5D12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. PP2A subunit B isoform B56 d (PPP2R5D) belongs to the phosphatase PP2A regulatory subunit B family. PP2A subunit B isoform B56 d (PPP2R5D) encodes a δ isoform of the regulatory subunit B56 subfamily. It is a member of phosphatase PP2A regulatory subunit B family. The core enzyme is a dimer composed of a catalytic subunit (C) and a constant regulatory subunit (A), that associates with a regulatory subunit (B).

Spezifität

The antibody recognizes human and rat PP2A B subunit isoform B56-δ. The antibody does not cross-react with PP2A-B55, PP2A-B56-α, PP2A-B56-β, PP2A-B56-γ or PP2A-B56-ε.

Immunogen

fusion protein corresponding to the N-terminus of human PP2A B subunit isoform B56δ.

Anwendung

Monoclonal Anti-PP2A subunit B isoform B56-δ antibody produced in mouse has been used in immunoblotting.[1]

Biochem./physiol. Wirkung

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells .

Protein phosphatase 2A (PP2A) is implicated in the negative control of cell growth and division.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable