R6379
Xho I from Xanthomonas holcicola
Restriction Enzyme
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About This Item
Empfohlene Produkte
Qualität
for molecular biology
Form
buffered aqueous glycerol solution
Konzentration
10,000 units/mL
Versandbedingung
wet ice
Lagertemp.
−20°C
Spezifität
Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Anwendung
XhoI is a resteriction endonuclease used for molecular biology methods to cut DNA molecules at the recognition sequence 5′-C/TCGAG-3′ to generate fragments with 5′-cohesive ends.
Sonstige Hinweise
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Physikalische Form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Polydocanol (v/v) at 4 °C
Ähnliches Produkt
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves
Analysenzertifikate (COA)
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A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum.
T R Gingeras et al.
Journal of molecular biology, 118(1), 113-122 (1978-01-05)
B Haribabu et al.
The Journal of experimental medicine, 192(3), 433-438 (2000-08-10)
Leukotrienes are derived from arachidonic acid and serve as mediators of inflammation and immediate hypersensitivity. Leukotriene B(4) (LTB(4)) and leukotriene C(4) (LTC(4)) act through G protein-coupled receptors LTB(4) receptor (BLTR) and Cys-LTR, respectively. To investigate the physiological role of BLTR
H Sesaki et al.
The Journal of cell biology, 152(6), 1123-1134 (2001-03-21)
Membrane fusion plays an important role in controlling the shape, number, and distribution of mitochondria. In the yeast Saccharomyces cerevisiae, the outer membrane protein Fzo1p has been shown to mediate mitochondrial fusion. Using a novel genetic screen, we have isolated
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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