I bought this kit as replacement of NA0410-1Kt which I was waiting for 1 year and still wasn't able to get due to delay. Plex15 is significantly more expensive, uncomfortable to work with and yields less plasmid DNA. I regret I had to waste my time and money on it.
PLEX15
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
sufficient for 15 purifications
GenElute™ Endotoxin-free Plasmid Maxiprep Kit
Synonym(e):
GenElute™ Endotoxin-free Plasmid Kits, Gen Elute
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1 KIT
549,00 €
549,00 €
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Über diesen Artikel
NACRES:
NA.52
UNSPSC Code:
41105501
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Unterstützung erhaltenusage
sufficient for 15 purifications
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
Quality Level
test parameters
: <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture
greener alternative category
, Aligned
storage temp.
15-25°C
1 of 4
Dieser Artikel | PLX15 | NA0300 | NA0410 |
|---|---|---|---|
| usage sufficient for 15 purifications | usage sufficient for 15 purifications | usage sufficient for 10 purifications | usage sufficient for 25 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
General description
The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 ug of endotoxin-free plasmid DNA may be prepared from 5-40 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
Features and Benefits
- High transfection efficiency and yield
- Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
- Fast and simple allowing up to 12 preps in less than 2 hours
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Lagerklasse
10 - Combustible liquids
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Verwandter Inhalt
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
Werkzeuge für die Plasmidpräparation, in vitro Transkription, mRNA-Reinigung und LNP-Formulierung in der mRNA-basierten Impfstoff- und Therapieentwicklung.
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