P3818

Protein-Disulfid-Isomerase aus Rinderleber

≥100 units/mg protein, lyophilized powder

• Synonym(e): PDI
• CAS-Nummer: 37318-49-3
• EC-Nummer: 5.3.4.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00163364
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥100 units/mg protein
• Mol-Gew.: 107 kDa
• Zusammensetzung: Protein, ~10% Lowry
• Lagertemp.: −20°C
• SMILES String: [S](=O)(=O)(ON)c1c(cc(c(c1)C(=O)O)Cl)Cl
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• InChIKey: DHUYKLYJBKXDBM-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2.[1] The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose.[2] PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.[3]

Anwendung

Protein Disulfide Isomerase from bovine liver has been used:

• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi[3]
• in the in vitro translation reaction for the generation of disulfide bonds[4]
• in insulin-disulfide reduction assay and peptide binding assay[5]
• as a positive control in thiol-disulfide oxidoreductase activity assay[6]

Biochem./physiol. Wirkung

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges.[4] It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.[7]

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.[8]

Verpackung

Package size based on protein content.

Physikalische Eigenschaften

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Einheitendefinition

One unit cause a change in A₆₅₀ of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

Physikalische Form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)